This opens the Settings window. olecular saka. Features. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 0 out of 5 $ 14,499 $ 13,995-13%. Brand: MOLECULAR DEVICES. 3 kg. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. Also refer to this Multimode Reader Comparison Chart. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 5°C at 37°C. 5,112,134 5,766,875. . SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. These measurements can include parameters such as absorbance, fluorescence intensity and. SpectraMax Quant dsDNA Assay Kits. spr SpectraMax Plus 384. flexibility of monochromator-based systems. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Capture flash assays with ease Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system withThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. . 4. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. This reader's dual monochromators for. Spectramax Multi-Mode Microplate Reader Performance Specifications. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Plate Shaking. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Perform the scan and note the wavelength that gives maximum emission. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Description 1. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Multimode Reader Comparison Chart. The SpectraMax iD3 reader detects fluorescence using monochromators only. of each sample medium was transferred to a 96-well flat-bottom plate. Product Information. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. The University of Rouen. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2/m2e user guide SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM SpectraMax M2 & SpectraMax…plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 0 out of 5 $ 3,895. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. The Cut-off filter for this pair is showing 530nm in this machine. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. E Models. Last lab had ours for years and never had a problem with it. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Three data types for FP read types are also being introduced with v4. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax Quant dsDNA Assay Kits. These lab workhorses are used in drug discovery. assays, we can offer a microplate reader solution to help meet the. 1. 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 47 and 0. D 59 A. 25 to 3. g. NanoQuant plate. Absorbance cuvette port. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. The SpectraMax® M2 and M2e Multimode. SpectraMax ® M2 Multi-Mode Microplate Reader. 4. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. Reviews. 2. Step 5: Click the software’s Read button. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. When a feature or capability applies to only certain readers, this exception is noted. 8: theMicroplate Reader & Luminometer Selection Guide. 1. 7. The fluorometric. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. The systems have. 1. Downloads. FI assay. variety of. 2. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. It does not work as expected. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The following general steps are taken with our absorbance and fluorescence readers. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 25 to 3. On microplate readers,. D 47 7. Download Application Note Plate reader settings for NanoOrange assay. ID: MOLECULAR DEVICES SpectraMax M2 is a multi-mode microplate spectrophotometer providing unbeatable accuracy, dependability and versatility for any lab experiment, with a range of detection modes, advanced accessories, and intuitive software. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 315 can't find zero order. 0 out of 5 $ 2,999. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Refurbished. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. 312 gain calibration failed. SpectraMax M2 and use thereof is covered by issued (U. Figure 1. Self Service Available. 1. 0) OD (0. 2018. 0077 OD 0. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 020 OD 2. SpectraTest FL1 Fluorescence Validation . ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Download PDF. 0 out of 5 $ 3,249. The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 1. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 3: The cuvette chamber. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. (See Figure 7. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. Type Clear: Add to Cart. S. 40266 Email: drugdiscoverytech@lifetech. 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 49 µM,. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. S. 0 out of 5 $ 9,995. SpectraMax ABS Plus SpectraTest ABS2 v1. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. For a higher degree of automation, the Automation. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. Add to Cart. CO 2 & O 2 control. Add to Cart. 53 - 60 kg, (117-132 lb), depending on configuration. Condition: Used - Good. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. the reader’s stated range. )Molecular Devices SpectraMax M2 Microplate Reader. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. spr SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. An assay plate containing a 15-point standard curve was read on the SpectraMax. This plate reader is capable of monitoring UV and visible absorbencies, fluorescence, and luminescence. 0 out of 5 $ 16,499. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. Plate chamber design of the SpectraMax category 1 microplate readers. The fluorescence was measured in the SpectraMax M3 Microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 53 - 60 kg, (117-132 lb), depending on configuration. BMG Labtech - THERMOstar. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. patent nos. Description 1. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. It can measure fluorecence from the top or bottom of a plate with a range of 250 - 850 nm. 67. It is particularly powerful when monitoring fast kinetic assays in a high. 0 out of 5. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 software was used to handle data analysis and control each instrument. Protein assays. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. $ 9,499. Selecting your. Plate Readers. Molecular Devices i3x. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Add to Cart. The Spectramax i3 is a muti-mode plate reader used for measuring absorbance and fluorescence in microtiter plates. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. After washing with PBS, the fluorescence intensities for each staining were analyzed with a fluorescence plate reader (SpectraMax M2). Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Bioz Stars score: 86/100, based on 1 PubMed citations. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. The SpectraTest fluorescence test plate (FL-1) can be used by Gemini XPS & M2 readers. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. FDSS µCell is a whole plate imager that measures fluorescence or luminescence from all wells of microplate continuously and simultaneously with built-in 96/384 channel liquid handling robotics. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Category 2 and 3 microplate readers use gradient heating as well. Sale. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. 0 × 103 to 2. Biotek Synergy 2 Plate Reader Multi-Mode; Related products. g. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. 0 × 107) RLU 0. Fluorescence Microplate Reader Comparison Chart. Highly selective for dsDNA over ssDNA or RNA. spr SpectraMax Plus 384 SpectraTest ABS2 v1. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The SpectraMax. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. D 3 1. Learn how you can utilize microplate readers to drive landmark discoveries. CA$14,500. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Molecular devices SpectraMax M3 Pdf User Manuals. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The drawing shows a standard microplate of 15 mm height covered with a lid. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. Appendix A. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these accurate, versatile microplate readers. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Microplate Formats: 6, 12, 24, 48, 96, 384. Uses dual. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Absorbance (monochromator). Automated live cell imaging – cell counting and confluence. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. 7°C (gradient heating design). SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax Spectrofluorometers. The calculated LOQ values were 0. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). We use Spectramax M5 microplate reader in our lab. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Figure 6: Transcreener ADP2 FI assay principle. BioTek’s Cytation™3, for instance, is a multimode plate reader that also functions as a high-speed automated digital fluorescence microscope with brightfield capability. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Reading Capabilities: cuvette or microplate. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. begin reading microplates with seven mouse clicks. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. B. Microscopes and Microplate Instrumentation. D 4/21/06 9:53:29 AM . Fluorescence read mode and Endpoint read type are already selected in the pre-configured protocol. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. Select the Driver tab and click Update Driver. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Fluorescence Microplate Readers 4. SpectraMax® Microplate Readers and SoftMax® Pro Software. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. Adjusting fluorescence gain on microplate readers. The chamber contains. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. Plate reader instructions Computer. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. Molecular Devices SpectraMax M2. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The instrument is located in room 2131 at 300 George Street. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The plate is set up for reading and images are acquired according to specified parameters. Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. A . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 7. I understand the OD measurement in a plate reader varies with the volume of the sample measured. The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. Category 2 and 3 microplate readers use gradient heating as well. 627. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. Offer Ends In: 45 Days 06: 20: 11. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. When a. e. Tecan’s. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. Microplate Reader, Multimode. 87. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 0 out of 5 $ 7,699. 100 readings per well gives optimal Z’ values for this assay. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. 00. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemFAST AND ACCURATE METERTECH Microplate Reader $3,650. Shop all used microplate readers and parts from a variety of manufacturers. 2. 2 only) SpectraMax M-series Microplate readers. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. 8 foot) 4400-0002 Power cord, 1 meter (3.